antibodies against ampkα, phosphorylated (p)ampkα (thr172), acc, p-acc (ser79), and β-actin  (Cell Signaling Technology Inc)

Journal: Nutrients

Article Title: Arene Substitutions in Orchid Bibenzyls: Mechanistic Insights into Glucose Uptake and Lipid Metabolism for Targeting Metabolic Disorders

doi: 10.3390/nu17071104

Figure Lengend Snippet: Impacts of batatasin III and gigantol on adipogenesis-mediated signaling pathways. The activation (phosphorylation denoted with prefix p-) levels of some signaling pathways (AMPK-ACC and AKT/GSK-3 β) triggered during early differentiation of 3T3-L1 cells treated with non-cytotoxic concentrations (5–25 μM) of batatasin III ( A – F ) or gigantol ( G – L ) for 48 h were assessed using Western blot analysis. Undifferentiated (UC) and differentiated (DC) cells treated with vehicle (0.5% ( v / v ) dimethyl sulfoxide) served as controls. Protein levels were quantified using ImageJ software and normalized to β-actin. Numerical data are presented as means ± SDs from three independent experiments. Lowercase letters indicate statistically significant differences among means within the same experimental condition. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test at α = 0.05.

Article Snippet: The primary antibodies included GLUT1, GLUT4, PPARγ, C/EBPα, FAS, FABP4, PLIN1, adiponectin, AKT, phosphorylated AKT (p-AKT; Ser473), AMPKα, phosphorylated AMPKα (p-AMPKα; Thr172), AMPKβ1/2, phosphorylated AMPKβ1 (p-AMPKβ1; Ser182), ACC, phosphorylated ACC (p-ACC; Ser79), GSK3β, phosphorylated GSK3β (p-GSK3β; Ser9), and β-Actin, all purchased from Cell Signaling Technology (Danvers, MA, USA), while LPL and SREBP-1c antibodies were sourced from Invitrogen (Waltham, MA, USA).

Techniques: Protein-Protein interactions, Activation Assay, Phospho-proteomics, Western Blot, Software

Journal: Food & Nutrition Research

Article Title: Weissella cibaria MG5285 and Lactobacillus reuteri MG5149 attenuated fat accumulation in adipose and hepatic steatosis in high-fat diet-induced C57BL/6J obese mice

doi: 10.29219/fnr.v65.8087

Figure Lengend Snippet: Lipogenic protein expression in epididymal adipose tissue (AT) of HFD-induced obese mice. ND: normal diet, HFD: high-fat diet. Following 8-weeks administration of LAB strains, protein expression in epididymal AT was analyzed by western blot analysis. (A) western blot image and (B) relative protein levels of PPAR-γ, CEBP/α, FAS, aP2, LPL, p-AMPK/AMPK, and p-ACC/ACC. Results are presented as the mean ± SD ( n = 6). Different letters indicate significant differences between means at p < 0.05 by Duncan’s multiple range test.

Article Snippet: Antibodies against peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT or enhancer-binding protein alpha (C/EBPα), fatty acid synthase (FAS), adipocyte protein 2 (aP2), acetyl-CoA carboxylase (ACC), phosphorylated ACC (p-ACC), Sterol regulatory element-binding proteins (SREBPs), AMP-activated protein kinase (AMPK), phosphorylated AMPK (p-AMPK), and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot

Journal: Food & Nutrition Research

Article Title: Weissella cibaria MG5285 and Lactobacillus reuteri MG5149 attenuated fat accumulation in adipose and hepatic steatosis in high-fat diet-induced C57BL/6J obese mice

doi: 10.29219/fnr.v65.8087

Figure Lengend Snippet: Expression of proteins associated with lipid metabolism in liver tissue in liver tissue of HFD-induced obese mice. ND: normal diet, HFD: high-fat diet. Following 8-weeks of LAB strains administration, protein expression in liver tissue was analyzed by western blot analysis. (A) western blot image and (B) relative protein levels of SREBP-1c, FAS, p-AMPK/AMPK, and p-ACC/ACC. Results are presented as the mean ± SD ( n = 6). Different letters indicate significant differences between means at p < 0.05 by Duncan’s multiple range test.

Article Snippet: Antibodies against peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT or enhancer-binding protein alpha (C/EBPα), fatty acid synthase (FAS), adipocyte protein 2 (aP2), acetyl-CoA carboxylase (ACC), phosphorylated ACC (p-ACC), Sterol regulatory element-binding proteins (SREBPs), AMP-activated protein kinase (AMPK), phosphorylated AMPK (p-AMPK), and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot